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Volume 35, Number 4, 1998, Pages 185-188
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The Practical Application of PCR for the Verification of Hop Variety

By Atsushi Murakami

Abstract
Kirin has developed a PCR (Polymerase chain reaction) assay to verify the variety and purity of hops. Quality control of the hops used in brewing is essential. As the chemical values obtained from each variety have changed over the years and recent varieties produce very similar chemical profiles, the conventional chemical analyses used for the verification of hop vari- ety have limitations for practical use. Thirty-eight typical hop varieties from around the world can be distinguished using this PCR assay. Contamination caused by mixing hop varieties can also be determined. The hop DNA used in PCR is unique to the variety and is unaf- fected by environmental conditions. As a large number of varieties can be distinguished by PCR, this will have practical applications for the detection of contamination. Thus, we have developed a practical and highly reliable means of verifying the variety and the purity of purchased hops.
Keywords: None.

 

Sintesis
SINTISIS Kirin ha desarrollado una prueba en PCR (Reaccion en cadena de polimerasas) para verificar la variedad y pureza de los lupulos. El control de calidad de los lupulos usados en el proceso de elaboracion de la cerveza esesencial. Los analisis convencionales usados para la verificacion de la variedad del lupulo tienen limitaciones para el uso practico, debido a que los valores quimicos obtenidos de cada variedad ha cambiado a traves de los anos y nuevas variedades producen perfiles quimicos bastante similares. Se pueden distinguir treinta y ocho variedades tipicas de lupulo alrededor del mundo usado la prueba PCR. Tambien puede ser detenninada la contaminacion causada por la mezcla de las difer- entes variedades de lupulo. EL DNA del lupulo usado en PCR es exclusivo para la variedades puede set distinguidas por la prueba PCR, esta tendra aplicaciones practico para la deteccion de contaminaci6n. Asi',hemos desarroliado un medio practico y altamente confiable de verificaci6n de la variedad y pueza de los lupulos comprados.